MICROBIAL IDENTIFICATION
Applications
  • Industrial QC
  • Reference Labs
  • Environment Studies
  • Veterinary
  • Research & Education
  • Microbial Ecology
  • Agriculture
  • Food & water
Description
Biolog Gen III

Bacteria, Yeast and Fungi Identification

The Biolog Microbial ID System can rapidly identify over 2,500 species of aerobic and anaerobic bacteria, yeasts and fungi. These easy to use systems provide reference laboratory quality identifications.

Biolog’s powerful carbon source utilization technology accurately identi?es environmental and pathogenic microorganisms by producing a characteristic pattern or “metabolic ?ngerprint” from discrete test reactions performed within a 96 well microplate. Culture suspensions are tested with a panel of pre-selected assays, then incubated, read and compared to extensive databases of environmental organisms, human pathogens, veterinary pathogens and plant pathogens.

The one-minute-per-sample set up is much simpler and faster than DNA sequencing and the automated pattern matching eliminates the need for training and expertise in gene sequence interpretation.

GEN III MicroPlate for microbial identification

    1. NO Gram stain
    2. NO pre-tests
    3. NO follow-on tests
    4. ONE panel for both GN & GP bacteria
    5. ONE minute set-up
    6. OVER 1350 species coverage

Biolog’s latest generation redox chemistry enables testing and identification of aerobic Gram-negative and Gram-positive bacteria in the same test panel. Gram stain and other pre-tests are no longer needed. The expanded GEN III database is designed to meet the needs of Biolog’s broad customer base covering diverse disciplines of microbiology.

All Biolog Microbial Identification Systems — manual, semi-automated or fully-automated — use the powerful new GEN III MicroPlate, allowing users to determine the most appropriate system to fit their current budget and level of throughput.

Automated

The OmniLog® ID System offers a simple automated process to meet the dynamic workflow demands of a wide range of microbiology applications. Even organisms requiring special incubation temperatures or environments are easily accommodated, ensuring accurate identifcation for a broad spectrum of Gram-negative and Gram-positive bacteria.

The OmniLog® ID System fully automates the process of microbial identi?cation by incubating, reading and interpreting results from up to 50 Biolog MicroPlates™ at a time. The system software provides directives to guide all steps of the testing process for each MicroPlate, including sample placement and removal and extended incubation when needed.

Additional MicroPlates can be loaded and processed anytime there is an open tray or identifcation has been completed on a previously entered MicroPlate. Up to date information for each MicroPlate, including results as they become available, are continuously visible on the system’s menu screen.

Including:

•    the OmniLog GEN III Data Collection Software, RetroSpect 2.0 Data
      Management Software, Electronic user guide, OmniLog reader/incubator,
      computer, printer, pipettor, turbidmeter, training and a one year warranty.
•    Databases are sold seperately
•    Combo Systems include OmniLog Phenotype MicroArray (PM) Software.

Semi Automated:


The MicroStation™ ID System is a versatile system, with the ability to identify and characterize a wide range of environmental and pathogenic organisms across diverse fields of microbiology. Using all Biolog databases, over 2650 species of bacteria, yeast and filamentous fungi can be identified.

The unique metabolic pattern generated by the organism is recorded and compared to hundreds of identification profiles in a corresponding Biolog Database. The versatile plate reader uses dual wavelength readings to quantify color reactions in the MicroPlate wells, adding consistency and accuracy when reading the reaction patterns.

Including:

•    MicroLog™ 3 Software, RetroSpect 2.0 Data Management Software,
      Electronic user guide, reader, pipettor, turbidmeter and a one year
      warranty.
•    Databases are sold separately

Manual:

Biolog’s MicroLog® manual microbial ID system is the ideal choice for testing environments with lower volume requirements or budget constraints. The system offers basic identification capability for aerobic Gram-negative and Gram-positive bacteria. GEN III MicroPlate results are read visually and entered into the MicroLog Software’s data entry screen for analysis and organism identification.

Including:

•    MicroLog™ Software, Electronic user guide, turbidmeter and a one year
      warranty.
•    Databases and multi-channel pipettor are sold separately

A simple, straightforward procedure

1. Isolate a pure culture on agar media
2. Prepare inoculum at specified cell density
3. Inoculate the Biolog MicroPlate
4. Incubate the plate, observe and enter the reaction pattern to obtain ID result

Phenotype MicroArrays for Microbial Cells

Phenotype MicroArray Technology is now available for applications with nearly all important species of bacteria and fungi. Simple protocols are available for testing nearly 2000 cellular phenotypes simultaneously.

Advantages:

With microbial cells


Determine gene functions.
•    Compare gene knock-out mutants to wild types.
•    Compare naturally-isolated strains with different genetic backgrounds.

Identify novel antimicrobial targets by finding genes unique to pathogenic microorganisms.
•    Find phenotypes present in pathogenic but not in non-pathogenic strains.
•    Find phenotypes present in pathogenic microbes but not in host cells
      (animal or plant).

Test antimicrobial targets and drug leads by comparative phenotyping.
•    Determine MOA of drug leads.
•    Compare phenotypic changes caused by target gene knockout versus drug addition.

Bioprocess improvement
•    Compare and analyze different generations of production strains.
•    QC fingerprint production strains.
•    Scan 2000 culture conditions simultaneously to optimize the growth medium for product yield.

General cell characterization
•    Determine metabolic properties of any microbe.
•    Determine drug/chemical sensitivities.

Available tests for microbial cells
•    PM-1 to PM-10    Metabolic tests for bacteria and fungi.
•    PM-11 to PM-20  Chemical sensitivity tests for bacteria.
•    PM-21 to PM-25  Chemical sensitivity tests for fungi.